Product Introduction: This reagent is a light yellow transparent liquid with a pungent odor. The upper layer is Tris hydrochloric acid buffer solution, pH>7.8， The lower layer is a phenolic phase with added antioxidant 8-hydroxyquinoline. This product is suitable for separating DNA.

Usage: Tissue lysis buffer, ensure a pH of around 8.0 (0.1 volume of 1M Tris HCl can be added, pH 8.0 can be adjusted to around 8.0), then add 0.5 volume of DNA extraction phenol reagent (let it stand, take the lower phenol phase for use. The upper layer is Tris HCl solution, to prevent phenol from contacting air and slow down phenol oxidation), 0.5 volume chloroform: isoamyl alcohol (24:1), shake for 10 seconds, and then ice bath for 15 minutes. After centrifugation, take the aqueous phase (upper layer) and add an equal volume of isopropanol to precipitate DNA. Rinse once with 75% ethanol, slightly dry, and dissolve in an appropriate amount of TE for later use.

matters needing attention: DNA extraction phenol reagents have strong corrosiveness and should be avoided from skin contact or inhalation as much as possible. If it turns red or brown, it indicates oxidation has occurred and cannot be used anymore.

Solubility: 10 mg/mL in water